The HiSeq X Five and Ten Systems have been discontinued. We will continue to provide full support of the instruments through March 31, 2024. To find an alternative instrument, we recommend using the Sequencing Platform Comparison Tool.
HiSeq X
Human WGS with the HiSeq X Five System
The HiSeq X Five System enables WGS at a cost and scale appropriate for large genome centers, and offers a scalable path to $1000 human genome capacity. With a lower initial capital investment, the HiSeq X Five System consists of 5 individual HiSeq X instruments, which together can sequence over 9000 genomes per year.
System Specifications
1.6–1.8 Tb
output range
5.3–6 billion
reads per run
2 × 150 bp
max read lengthTrade in your HiSeq instrument for more discovery power.
If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.
Customer Stories
Our Lives in Data Exhibit at the London Science Museum
The Illumina HiSeq X instrument is being displayed as an example of how sequencing technology is helping amass the data in the 100,000 Genomes Project, allowing scientists to do pioneering research.
View VideoUnmasking Autoimmune Diseases Using Genomics
Researchers are performing WGS and WES to identify variants associated with lupus and other autoimmune disorders.
Read articleWitnesses to a Sea Change in Sequencing Capability
Three luminaries provide their perspective on the impact of high-throughput and population sequencing in clinical research and the role they will play in the future of medicine.
Read article
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Featured Products
cBot 2 System (discontinued)
The cBot 2 System streamlines the sequencing workflow by automating cluster generation. It is a required accessory instrument for HiSeq sequencers.
HiSeq X Reagent Kits
Kits include SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
TruSeq DNA PCR-Free
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.