Cancer Whole-Exome Sequencing
Identifying Coding Mutations in Cancer
Sequencing the cancer exome provides useful information about the coding mutations that contribute to tumor progression. Because the exome represents less than 2% of the genome, cancer exome sequencing using next-generation sequencing (NGS) technology is a cost-effective alternative to whole-genome sequencing. Exome sequencing also produces a more manageable data set compared to whole-genome approaches.
Exome Sequencing of Melanoma and Colon Cancer Samples
Researchers using Illumina sequencing identified JAK1/2 mutations in tumors which were not responsive to PD-1 blockade.
Key benefits of cancer exome sequencing
- Identifies cancer variants across a wide range of applications
- Offers a cost-effective alternative to whole-genome sequencing
- Enables deep sequencing of coding regions of the genome to detect rare cancer-associated variants
- Generates a more manageable data set for easier analysis than WGS data
Featured Cancer Exome Sequencing Research
NGS Proves Invaluable for Biomarker Discovery
Cancer researchers use exome, RNA, and chromatin immunoprecipitation sequencing methods in their search for tumor-associated gene expression profiles.
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Capturing Variant Data from Blood
Cancer researchers use whole-exome, whole-genome, and transcriptome sequencing of exosomal DNA and RNA from pancreatic cancer samples to gain insight into the potential of liquid biopsies.
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Exploring the Genetic Basis of Oral Cancer
Researchers use a variety of methods, including whole-exome sequencing, to study a deadly oral cancer. They have identified somatic variants and other genomic alterations that may influence the course of the disease.
Read ArticleFeatured Cancer Exome Sequencing Research
NGS Proves Invaluable for Biomarker Discovery
Cancer researchers use exome, RNA, and chromatin immunoprecipitation sequencing methods in their search for tumor-associated gene expression profiles.
Read ArticleExploring the Genetic Basis of Oral Cancer
Researchers use a variety of methods, including whole-exome sequencing, to study a deadly oral cancer. Dr. Majumder and his team have identified somatic variants and other genomic alterations that may influence the course of the disease.
Read ArticleComprehensive cancer whole-exome sequencing workflow
Illumina offers library preparation, sequencing, and data analysis research options for sequencing cancer exomes. Streamlined library prep workflows and flexible kit configurations accommodate multiple study designs. User-friendly tools simplify data analysis.
Library prep
Illumina DNA Prep with Exome 2.5 Enrichment
A high-performance, fast, and complete whole-exome sequencing kit including library prep and hybridization reagents, a comprehensive exome probe panel, clean up/size selection beads, and indexes.
Sequencing
NextSeq 1000 and 2000 Systems
These cost-efficient, user-friendly, mid-throughput benchtop sequencers offer extreme flexibility to support new and emerging applications.
NovaSeq X Series
The NovaSeq X Series offers vast application breadth, enabling data-intensive methods at production scale.
Data analysis
Illumina DRAGEN Secondary Analysis
Accurate, comprehensive, and efficient analysis of NGS data for a range of applications, including exome as well as genome, transcriptome, and methylome studies.
Related sequencing methods
Cancer whole-genome sequencing
Discover novel features and benefits with whole-genome sequencing for cancer research.
Cancer RNA sequencing
See how RNA-Seq can reveal information about cancer gene expression and gene fusions that drive tumor progression.
Cancer Research Methods Guide
The Cancer Research Methods Guide is a 40+ page comprehensive resource with simple, comprehensive workflows for a broad range of cancer research applications. This guide includes single-cell sequencing, spatial sequencing, methylation profiling, multiomics, cell-free RNA sequencing, and more.
Download free guideKeep exploring
Tackling the unique complexity of pediatric cancer
A new exome-based test will help determine the genetic variants—germline and somatic—driving these rare cancers.
NGS proves invaluable for finding rare biomarkers
In this article, Dr. Vivek Subbiah explains how finding a rare biomarker can have a big impact on cancer patients.
Why choose whole-genome or exome sequencing?
Explore the benefits of both approaches to understand which method is best for your research.
References
Schwarze K, Buchanan J, Taylor JC, Wordsworth, S. Are whole-exome and whole-genome sequencing approaches cost-effective? A systematic review of the literature. Genet Med. 2018 Oct;20(10):1122-1130. doi: 10.1038/gim.2017.247.