HiSeq X Reagent Kits
HiSeq X System
Reagent Kits
Catalog IDs: FC-501-2501, FC-501-2521, FC-502-2501, FC-502-2521
Kit Requirements
Performing a sequencing run requires a single-use HiSeq X Ten Reagent Kit v2.5 or HiSeq X Five Reagent Kit v2.5. Each kit is available in two bundles:
- Single-pack kit—Provides flow cells, reagents, and accessories for one dual flow cell run or two single flow cell runs.
- Ten-pack kit—Provides flow cells, reagents, and accessories for 10 dual flow cell runs or 20 single flow cell runs.
When preparing consumables, check the coded labels:
- PE cluster kit—Green rectangle marked PE
- Flow cells—Green rectangle
- SBS kit box—Blue dot
For other system requirements and compatible products, see HiSeq X Compatibility & Requirements.
Contents and Storage
Single-Pack Kit
The single-pack kit contains the following components. Store at the temperatures indicated in the following tables.
Quantity |
Component |
|
| 2 | PE Cluster Kit v2 (Box 1 of 2) | |
| 2 | PE Cluster Kit v2 (Box 2 of 2) | |
| 2 | Patterned Flow Cell | |
| 2 | cBot Manifold | |
| 2 | SBS Kit v2 (Box 1 of 2) | |
| 2 | SBS Kit v2 (Box 2 of 2) | |
| 2 | Accessory Kit v2 |
PE Cluster Kit Box 1, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 1 | cBot Reagent Plate | cBot Reagent Plate, PE |
| 1 | RSB | Resuspension Buffer |
| 1 | EXP1 | Enhanced Patterned Cluster Mix 1 |
| 1 | EXP2 | Enhanced Patterned Cluster Mix 2 |
| 1 | EXP3 | Enhanced Patterned Cluster Mix 3 |
PE Cluster Kit Box 2, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 1 | PRM | Oncology RNA Probes Master Pool |
| 1 | PLM v2 | Patterned Linearization Mix 2 |
| 1 | PAM | Patterned Amplification Mix |
| 1 | PPM | Patterned Amp Premix |
| 1 | PDR | Patterned Denaturation Mix (contains formamide) |
| 1 | HP11 | Read 2 Primer Mix |
| 1 | HP14 | Index i5 and i7 Primer Mix |
SBS Kit Box 1
Quantity |
Reagent |
Description |
Storage |
| 1 | PW1 | Wash Buffer | -25°C to 30°C |
| 1 | PB1* | Patterned SBS Buffer 1 | 15°C to 30°C |
| 3 | PB2 | Patterned SBS Buffer 2 | 15°C to 30°C |
* PB1 is sensitive to light. Store PB1 in a dark location.
SBS Kit Box 2, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 1 | PCM | Patterned Cleavage Mix |
| 1 | PIM* | Patterned Incorporation Mix |
| 1 | PSM | Patterned Scan Mix |
* PIM is sensitive to light. Store PIM in a dark location.
Accessory Kit, Store at Room Temperature
Quantity |
Accessory |
Purpose |
| 1 | Flow cell gaskets (package of 4) |
Replace the flow cell gaskets between runs |
| 1 | Flow cell storage tube with storage buffer, 50 ml | Store the flow cell between clustering and sequencing |
| 1 | Funnel bottle caps (package of 8) |
Replace the caps on SBS reagent bottles before loading reagents |
Ten-Pack Kit
The 10-pack kit contains the following components. Store at the temperatures indicated in the following tables.
Quantity |
Component |
|
| 1 | PE Cluster Kit v2 (Box A) | |
| 1 | PE Cluster Kit v2 (Box B) | |
| 1 | PE Cluster Kit v2 (Box C) | |
| 1 | Patterned Flow Cell (Box of 20) | |
| 20 | cBot Manifold | |
| 1 | SBS Kit v2 (Box A) | |
| 2 | SBS Kit v2 (Box B) | |
| 6 | SBS Kit v2 (Box C) | |
| 2 | SBS Kit v2 (Box D) | |
| 2 | SBS Kit v2 (Box E) | |
| 2 | SBS Kit v2 (Box F) | |
| 1 | Accessory Kit v2 (Box A) | |
| 1 | Accessory Kit v2 (Box B) |
PE Cluster Kit Box A, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 20 | cBot Reagent Plate | cBot reagent plate, PE |
PE Cluster Kit Box B, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 5 | RSB | Resuspension Buffer |
| 5 | EPX1M | Enhanced Patterned Cluster Mix 1 |
| 5 | EPX2M | Enhanced Patterned Cluster Mix 2 |
| 5 | EPX3M | Enhanced Patterned Cluster Mix 3 |
Enhanced Patterned Cluster Mix 1 |
Enhanced Patterned Cluster Mix 1 |
PE Cluster Kit Box C, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
| 20 | PRM | Oncology RNA Probes Master Pool |
| 20 | PLM v2 | Patterned Linearization Mix 2 |
| 20 | PAM | Patterned Amplification Mix |
| 20 | PPM | Patterned Amp Premix |
| 20 | PDR | Patterned Denaturation Mix (contains formamide) |
| 20 | HP11 | Read 2 Primer Mix |
| 20 | HP14 | Index i5 and i7 Primer Mix |
SBS Kit Boxex A-F
Box |
Reagent |
Quantity |
Description |
Storage |
| SBS Box A | PW1 | 20 each | Wash Buffer | -25°C to 30°C |
| SBS Box B | PB1* | 10 each | Patterned SBS Buffer 1 | 15°C to 30°C |
| SBS Box C | PB2 | 10 each | Patterned SBS Buffer 2 | 15°C to 30°C |
| SBS Box D | PCM | 10 each | Patterned Cleavage Mix | -25°C to 30°C |
| SBS Box E | PIM* | 10 each | Patterned Incorporation Mix | -25°C to 30°C |
| SBS Box F | PSM | 10 each | Patterned Scan Mix | -25°C to 30°C |
* PB1 and PIM are sensitive to light. Store in a dark location.
Accessory Kit Boxes A and B, Store at Room Temperature
Box |
Accessory |
Quantity | Purpose |
| Box A | Flow cell storage tube with storage buffer, 50 ml | 20 each | Replace the flow cell gaskets between runs. |
| Box B | Flow cell gaskets (package of 4) |
20 each | Store the flow cell between clustering and sequencing. |
| Box B | Funnel bottle caps (package of 8) |
20 each | Replace the caps on SBS reagent bottles before loading reagents. |